Amino Acid Composition of Melagenin Plus®

Abstract

Introduction: Melagenin Plus® is a hydroalcoholic extract of human placenta successfully employed for the treatment of vitiligo.

Objective: To independently determine the free and total amino acids, as well as the amino acid profile of Melagenin Plus®, using the amino acid analysis technique.

Methods: After derivatization, amino acids were separated and quantified by gas chromatography. A three-level concentration curve was prepared containing a mixture of the standard amino acids to be identified; quantification was performed by the internal standard method.

Results: A total of 15 amino acids were identified: alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, glycine, serine, threonine, tyrosine, aspartic acid, glutamic acid, histidine and lysine. 2,163 mg/100 mL ± 0.352 of free form amino acids and 4,513 mg/100 mL ± 3,047 of total amino acids were quantified.

Conclusions: Glutamic acid had the highest representation in the product. The other most abundant amino acids were glycine, methionine, serine, proline and leucine.